What is ONPG and what is it used for?

ONPG, or 2-Nitrophenyl-B-D-Galactopyranoside, is a synthetic compound widely used in biochemical research. It serves as a substrate in enzyme assays, particularly for detecting the activity of the enzyme beta-galactosidase. When cleaved by this enzyme, ONPG produces a yellow chromophore, allowing for quantitative measurement. Its application is common in microbiology labs for studying lactose metabolism in various organisms.

What are the storage conditions for ONPG?

ONPG should be stored at a temperature ranging from 2 to 8 degrees Celsius, protected from light. Proper storage is crucial to maintain its stability and activity, preventing degradation. Ensure the container is tightly sealed to avoid moisture absorption. This practice helps maintain the integrity of the compound for accurate experimental results in enzymatic assays.

How is ONPG typically used in laboratory experiments?

In laboratory settings, ONPG is primarily used in assays to detect beta-galactosidase activity. Researchers prepare a reaction mix that includes ONPG and the biological sample suspected of producing the enzyme. After incubation, the intensity of the yellow color developed is measured using a spectrophotometer, indicating the enzyme’s activity level, which is critical in various biochemical analyses.

Is ONPG safe to handle in the lab?

While ONPG is generally considered safe, it should be handled with care, using appropriate personal protective equipment, such as gloves and goggles. Accidental ingestion or skin exposure should be avoided. As with all chemicals, consult the Safety Data Sheet (SDS) for information on hazards and safe handling procedures to minimize risks and ensure a safe working environment.

Can ONPG be used in urgent scientific testing?

Yes, ONPG can be used in urgent scientific testing, especially in microbiological studies requiring fast and reliable results for lactose fermentation assays. Its quick reaction time allows researchers to obtain actionable data rapidly, facilitating timely decision-making in experiments and studies focused on lactose metabolism in various pathogens or cell cultures.

What reactions can ONPG undergo in the presence of beta-galactosidase?

In the presence of beta-galactosidase, ONPG undergoes hydrolysis, resulting in the release of galactose and 2-nitrophenol. This reaction produces a yellow color, which can be tracked to measure enzyme activity. This property enables researchers to determine the ability of various microorganisms to utilize lactose, making ONPG a valuable tool in assessing microbial metabolism.

What are the typical applications of ONPG in industry?

In industry, ONPG is commonly applied in microbiological quality control, particularly in the dairy and food industry, where lactose fermentation must be monitored. It is used to assess the capability of lactose-utilizing bacteria in fermented products. Moreover, ONPG assists in various molecular biology applications to understand genetic expression related to lactose metabolism.

How can I confirm the quality or purity of ONPG?

To confirm the quality or purity of ONPG, researchers can perform a spectrophotometric analysis to measure absorbance characteristics that correspond to pure samples. Additionally, comparing the performance of curves and reaction times against standard benchmarks can ensure its reliability in assays, enabling accurate and reproducible data in scientific experiments.

What is the shelf life of ONPG after opening?

The shelf life of ONPG after opening generally depends on storage conditions, and it is advisable to use it within a few months for optimal results. To maintain stability, store it properly at low temperatures and avoid exposure to light. Regularly checking for color changes in the solution can help assess its viability for ongoing experiments.

Where can I buy ONPG 2-Nitrophenyl-B-D-Galactopyranoside, 5 Grams?

You can buy ONPG 2-Nitrophenyl-B-D-Galactopyranoside, 5 Grams at Ubuy in Montserrat. Ubuy offers a reliable platform for purchasing laboratory materials, ensuring you access quality products for your scientific needs quickly and conveniently.

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Is [ONPG] 2-Nitrophenyl-B-D-Galactopyranoside, 5 Grams Available to Shop Online in Montserrat?

Yes, at Ubuy Montserrat this product is available for you to shop at a reasonable price. The [ONPG] 2-Nitrophenyl-B-D-Galactopyranoside, 5 Grams is not available locally but you can trust us with our express shipping services.

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RPI

[ONPG] 2-Nitrophenyl-B-D-Galactopyranoside, 5 Grams

High purity and solubility make it indispensable for research applications.

RPI

Item #: 81622265

[ONPG] 2-Nitrophenyl-B-D-Galactopyranoside, 5 Grams

81% of respondents would recommend this to a friend

Item #: 81622265

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High purity and solubility make it indispensable for research applications.

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What Stands Out

High Purity

This 2-Nitrophenyl-B-D-Galactopyranoside offers exceptional purity, ensuring reliable results for biochemical assays and reactions, outperforming competitor products in accuracy and consistency.

Versatile Applications

Ideal for enzyme assays and research purposes, this compound serves multiple scientific applications, making it a valuable addition for laboratory work in microbiology and biochemistry.

Easy Handling

Packaged in a convenient 5-gram size, it provides ease of use for researchers, minimizing waste while allowing precise measurements for experiments, enhancing the overall lab efficiency.

Product Details

% Assay of Chemical(s)	99
Item Weight	27.22 g
Brand Name	RPI
Manufacturer	RPI, Corp.
Part Number	N81000-5.0
Brand	RPI
Percentage assay	99

Who Should Buy?

Biochemistry Students
Ideal for students studying enzyme kinetics and carbohydrate metabolism in laboratory settings requiring high-quality substrates.
Research Laboratories
Useful for microbiology and biochemistry research focusing on lactose utilization and enzyme activity tests.
Educational Institutions
Perfect for institutions conducting practical experiments in enzyme activity, aiding learning of enzyme-substrate interactions.

Casual Users
Not suitable for non-scientific users or hobbies lacking a clear need for biochemical reagents.

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Customer Questions & Answers

Question: How to Shop [ONPG] 2-Nitrophenyl-B-D-Galactopyranoside, 5 Grams Online From Ubuy?

Answer: It’s easy to shop [ONPG] 2-Nitrophenyl-B-D-Galactopyranoside, 5 Grams online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is [ONPG] 2-Nitrophenyl-B-D-Galactopyranoside, 5 Grams Available to Shop Online in Montserrat?

Answer: Yes, at Ubuy Montserrat this product is available for you to shop at a reasonable price. The [ONPG] 2-Nitrophenyl-B-D-Galactopyranoside, 5 Grams is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

RPI Standards Editorial Review

[ONPG] 2-Nitrophenyl-B-D-Galactopyranoside, 5 Grams is a high-purity chemical with an assay percentage of 99%. Manufactured by RPI, this compound is widely used in biochemical research and applications. The reliability of this product is emphasized by its precise composition which makes it essential for various experimental protocols. Researchers appreciate the high assay level that ensures accuracy in their work. The item weighs 27.22 grams, making it easy to handle and store. Lab professionals recommend this product for reliable and consistent results in their experiments, contributing to its positive reception among users.

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Pros

High purity with 99% assay for reliability
Lightweight at 27.22g for easy handling
Manufactured by reputable RPI brand
Essential for various biochemical applications
Precision in research protocols

Cons

Only available in 5 grams size

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Features & Benefits

High chemical purity of over 99%.
Suitable for colorimetric and spectrophotometric detection.
Specifically designed for detecting β-D-Galactosidase.
Soluble in DMSO and DMFO for versatile use.
Ideal for research and further manufacturing applications.
Not intended for food or drug use.

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[ONPG] 2-Nitrophenyl-B-D-Galactopyranoside, 5 Grams