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72445879
10% Sodium Dodecyl Sulfate (SDS) Solution - DNase/RNase/Protease Free SDS in Autoclaved 18.2 Ohm Deionized Water - 125mL
89% of respondents would recommend this to a friend
Item #:
72445879
XCD 144
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UFC Bio's 10% SDS solution is made with DNase, RNase, Protease free SDS and autoclaved 18.2Ω deionized water.
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What Stands Out
High Purity
This SDS solution is free from DNase, RNase, and protease, ensuring no contamination which is critical for sensitive experiments.
Autoclaved Solution
The 125mL solution is autoclaved, providing sterile conditions that enhance reliability for laboratory applications.
Ideal Concentration
A 10% concentration in 18.2 Ohm deionized water makes it perfect for various biochemical applications, offering versatility and effectiveness.
Product Details
| Package Weight | 1.27 Pound |
Who Should Buy?
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Molecular Biologists
Ideal for molecular biology applications requiring SDS for protein or nucleic acid analysis without contamination risks.
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Laboratory Technicians
Perfect for technicians needing a reliable, sterile SDS solution for various biochemical and analytical procedures.
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Research Institutions
Suitable for research settings that prioritize purity and precision in experiments involving complex biological samples.
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General Cleaning
Not advised for general cleaning purposes as it is a specialized solution intended for laboratory applications.
Product Description
10% Sodium Dodecyl Sulfate (SDS) Solution - DNase/RNase/Protease Free SDS in Autoclaved 18.2 Ohm Deionized Water - 125mL
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Customer Questions & Answers
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Question:
What is Sodium Dodecyl Sulfate (SDS) used for in laboratories?
Answer: Sodium Dodecyl Sulfate (SDS) is commonly used as a surfactant and detergent in various laboratory applications, especially in the analysis of proteins. It helps to denature proteins, allowing them to be separated by their molecular weight through SDS-PAGE. This process is crucial for protein characterization, purification, and analysis, making SDS an essential reagent for biochemists and molecular biologists. -
Question:
What does it mean that the SDS solution is DNase, RNase, and Protease free?
Answer: The term DNase, RNase, and Protease free indicates that the SDS solution is purified to remove these enzymes, which can degrade nucleic acids and proteins. This level of purity is critical in experiments where the integrity of DNA, RNA, or proteins must be maintained, such as cloning, sequencing, or protein studies. Using this highly purified SDS ensures that your experimental results are accurate and reliable. -
Question:
What is the significance of using autoclaved deionized water in SDS solutions?
Answer: The use of autoclaved 18.2 Ohm deionized water in the preparation of SDS solutions ensures that the water is free from contaminants and microbial life. This high purity level is essential for laboratory experiments, particularly in molecular biology, to prevent interference from impurities that could affect the outcomes. Utilizing deionized water also contributes to the overall consistency and efficiency of experimental protocols. -
Question:
How should I store 10% SDS solution for optimal preservation?
Answer: To maintain the stability of the 10% SDS solution, store it at room temperature in a tightly sealed container, away from direct sunlight. Ensure that the solution is kept free from contaminants by using sterilized materials during handling. Proper storage is vital for preserving its effectiveness for experiments, preventing degradation or contamination that could otherwise alter experimental results. -
Question:
Can 10% SDS solution be used for protein extraction?
Answer: Yes, 10% SDS solution is commonly used for protein extraction. It helps disrupt cell membranes and solubilizes proteins, making it an effective reagent for isolating proteins from biological samples. This application is particularly useful in studies focused on protein analysis, where proteins need to be extracted in their denatured form for subsequent characterization like electrophoresis or western blotting. -
Question:
Is 10% SDS solution safe to handle in the laboratory?
Answer: While 10% SDS solution is a widely used laboratory reagent, it can be irritating to the skin, eyes, and respiratory system. Always handle it with care, wearing appropriate personal protective equipment such as gloves and goggles. Proper lab protocols and safety guidelines should be followed to minimize exposure, ensuring a safe working environment during your experiments. -
Question:
What applications benefit from using 10% SDS in biochemical experiments?
Answer: 10% SDS is beneficial in a variety of biochemical applications, including proteomics, enzyme activity assays, and nucleic acid analysis. It is particularly important for methods like SDS-PAGE, where it helps separate proteins based on size. Additionally, it is utilized in buffer preparations and as a part of lysis buffers, making it a versatile tool for researchers in molecular biology and biochemistry. -
Question:
What are the common concentrations for SDS solutions?
Answer: Sodium Dodecyl Sulfate solutions typically come in various concentrations, with 1%, 2%, and 10% being common. The selection of concentration depends on the specific application, with 10% being frequently chosen for denaturing proteins in SDS-PAGE. Researchers may adjust concentrations based on experimental needs, ensuring optimal conditions for their analyses and procedures. -
Question:
How do I prepare a 10% SDS solution from powder?
Answer: To prepare a 10% SDS solution, dissolve 10 grams of SDS powder in distilled water to make a total volume of 100 mL. It's important to add the SDS to the water gradually, ensuring complete dissolution. This preparation method ensures that your SDS solution is adequately mixed for effective use in your experiments. Remember to work in a suitable environment and use protective gear during the preparation. -
Question:
Where can I buy 10% Sodium Dodecyl Sulfate SDS Solution?
Answer: You can purchase 10% Sodium Dodecyl Sulfate SDS Solution - DNaseRNaseProtease Free SDS in Autoclaved 18.2 Ohm Deionized Water - 125mL from Ubuy. They provide a variety of laboratory supplies that can meet your research needs, ensuring quality and reliable materials for your scientific experiments.
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Pros
- High purity SDS solution
- DNase, RNase, protease free
- Convenient ready-to-use format
- Stable and reliable performance
- Compatible with various applications
Cons
- Occasional minor sedimentation
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Features & Benefits
- 10% SDS solution for research purposes only.
- Filtered through a 0.2um filter.
- Sterile solution made with DNase, RNase, Protease-free SDS.
- Used in gel electrophoresis applications.
- Helps determine a protein's molecular weight.
- Sample CoA in photos, CoA/SDS given upon request.
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